San Francisco-based startup Daytona has secured $60 million in a Series B funding round, reaching a valuation of $1 billion. The company, founded in 2023, provides cloud infrastructure specifically designed for AI agents to execute code and manage workflows in isolated, elastic environments.

The funding round was led by FirstMark, which previously led the company's $24 million Series A. Other participants included founders from Stripe, Shopify, Perplexity, Quora, and Zoom, alongside strategic investors such as Qualcomm Ventures, Samsung Next, and Okta Ventures.

Daytona previously raised approximately $2 million in a pre-seed round in 2023 and $5 million in a seed round in 2024. The company currently employs roughly 30 people and operates in both the US and Croatia.

The startup reported reaching $1 million in forward annual recurring revenue within 50 days of launch and $3 million in ARR within 95 days of pivoting to agent-native infrastructure. Its client base includes Meta, Cohere, and various Fortune 100 enterprises.